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acot7 rabbit polyclonal antibody  (Proteintech)


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    Proteintech acot7 rabbit polyclonal antibody
    Fig. 1 Identification of convergent evolutionary sites in flight-degenerate birds. a A phylogeny tree5 with American alligator as the outrgoup showing the convergence of eight studied flight-degenerate bird species. b Flying and flight-degenerate species were classified based on previous literatures and observations (Methods). Flight-degenerate species includes flightless birds and weak flyers. Average body mass of each species is plotted against its average wingspan measurement. c Two-dimensional frequency spectrum for nucleotides in flying and flight-degenerate species. The nucleotide frequencies of flight-degenerate birds were plotted against those of flying birds. The x-axis means the nucleotide counts among the eight flight-degenerate bird species and the y-axis means the nucleotide counts among 40 flying bird species. The number of loci is color-coded according to the scale on the right. d The pathway in which ATGL and <t>ACOT7</t> are involved35,70. The main pathway for lipid metabolism is shadowed. In e and f, the upper shows the significant values (Fisher’s exact test) for the representative nucleotides on ATGL e and ACOT7 f, the middle shows the sequence of amino acids, and the bottom shows the schematic domains. The identified convergent sites are highlighted in red. Source data are provided as a Source Data file
    Acot7 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acot7+rabbit+polyclonal+antibody/ACOT7+Antibody/pm31227702-222-14-19
    Average 93 stars, based on 10 article reviews
    acot7 rabbit polyclonal antibody - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Convergent genomic signatures of flight loss in birds suggest a switch of main fuel."

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.

    Journal: Nature communications

    doi: 10.1038/s41467-019-10682-3

    Fig. 1 Identification of convergent evolutionary sites in flight-degenerate birds. a A phylogeny tree5 with American alligator as the outrgoup showing the convergence of eight studied flight-degenerate bird species. b Flying and flight-degenerate species were classified based on previous literatures and observations (Methods). Flight-degenerate species includes flightless birds and weak flyers. Average body mass of each species is plotted against its average wingspan measurement. c Two-dimensional frequency spectrum for nucleotides in flying and flight-degenerate species. The nucleotide frequencies of flight-degenerate birds were plotted against those of flying birds. The x-axis means the nucleotide counts among the eight flight-degenerate bird species and the y-axis means the nucleotide counts among 40 flying bird species. The number of loci is color-coded according to the scale on the right. d The pathway in which ATGL and ACOT7 are involved35,70. The main pathway for lipid metabolism is shadowed. In e and f, the upper shows the significant values (Fisher’s exact test) for the representative nucleotides on ATGL e and ACOT7 f, the middle shows the sequence of amino acids, and the bottom shows the schematic domains. The identified convergent sites are highlighted in red. Source data are provided as a Source Data file
    Figure Legend Snippet: Fig. 1 Identification of convergent evolutionary sites in flight-degenerate birds. a A phylogeny tree5 with American alligator as the outrgoup showing the convergence of eight studied flight-degenerate bird species. b Flying and flight-degenerate species were classified based on previous literatures and observations (Methods). Flight-degenerate species includes flightless birds and weak flyers. Average body mass of each species is plotted against its average wingspan measurement. c Two-dimensional frequency spectrum for nucleotides in flying and flight-degenerate species. The nucleotide frequencies of flight-degenerate birds were plotted against those of flying birds. The x-axis means the nucleotide counts among the eight flight-degenerate bird species and the y-axis means the nucleotide counts among 40 flying bird species. The number of loci is color-coded according to the scale on the right. d The pathway in which ATGL and ACOT7 are involved35,70. The main pathway for lipid metabolism is shadowed. In e and f, the upper shows the significant values (Fisher’s exact test) for the representative nucleotides on ATGL e and ACOT7 f, the middle shows the sequence of amino acids, and the bottom shows the schematic domains. The identified convergent sites are highlighted in red. Source data are provided as a Source Data file

    Techniques Used: Sequencing

    Fig. 4 Modeling simulations of effects of functional changes on the energy landscape. The actual abundance of acyl-CoA a and acetyl-CoA b was measured in three flight-degenerate (turkey, chicken and ostrich) and three flying species (zebra finch, parrot and pigeon) using LC–MS. The simulated results for ATGL and ACOT7 are shown in c and d respectively. *P < 0.05, ***P < 0.001 by Wilcoxon rank sum test. Error bars indicate s.e.m. Source data are provided as a Source Data file
    Figure Legend Snippet: Fig. 4 Modeling simulations of effects of functional changes on the energy landscape. The actual abundance of acyl-CoA a and acetyl-CoA b was measured in three flight-degenerate (turkey, chicken and ostrich) and three flying species (zebra finch, parrot and pigeon) using LC–MS. The simulated results for ATGL and ACOT7 are shown in c and d respectively. *P < 0.05, ***P < 0.001 by Wilcoxon rank sum test. Error bars indicate s.e.m. Source data are provided as a Source Data file

    Techniques Used: Functional Assay, Liquid Chromatography with Mass Spectroscopy

    Related Articles

    Blocking Assay:

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel
    Article Snippet: .. After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat anti-rabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin). .. The differentiated mouse 3T3-L1 adipocyte cells expressed with pCDH-fACOT7 , pCDH-fACOT7mut , pCDH-fATGL , pCDH-fATGLmut or pCDH were collected in a 1.5 ml Eppendorf tube.

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.
    Article Snippet: .. After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat antirabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin). ..

    Incubation:

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel
    Article Snippet: .. After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat anti-rabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin). .. The differentiated mouse 3T3-L1 adipocyte cells expressed with pCDH-fACOT7 , pCDH-fACOT7mut , pCDH-fATGL , pCDH-fATGLmut or pCDH were collected in a 1.5 ml Eppendorf tube.

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.
    Article Snippet: .. After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat antirabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin). ..

    Control:

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel
    Article Snippet: .. After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat anti-rabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin). .. The differentiated mouse 3T3-L1 adipocyte cells expressed with pCDH-fACOT7 , pCDH-fACOT7mut , pCDH-fATGL , pCDH-fATGLmut or pCDH were collected in a 1.5 ml Eppendorf tube.

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.
    Article Snippet: .. After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat antirabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin). ..

    Chromatin Immunoprecipitation:

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used ATGL antibody (Cell Signaling Technology, 2138S), α-Tubulin (Abcam, ab7291), goat anti-rabbit antibody (LI-COR Biosciences, 926-68071), donkey anti-mouse antibody (LI-COR Biosciences, 926-32212), ACOT7 rabbit polyclonal antibody (Proteintech, 15972-1-AP), β-Actin mouse monoclonal antibody (Cell Signaling Technology, 8H10D10), goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology, sc-2004), and donkey anti-mouse IgG-HRP (Abcam, ab97030). .. Validation The ATGL antibody was obtained from Cell Signaling Technology and α-Tubulin from Abcam.

    Flow Cytometry:

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used ATGL antibody (Cell Signaling Technology, 2138S), α-Tubulin (Abcam, ab7291), goat anti-rabbit antibody (LI-COR Biosciences, 926-68071), donkey anti-mouse antibody (LI-COR Biosciences, 926-32212), ACOT7 rabbit polyclonal antibody (Proteintech, 15972-1-AP), β-Actin mouse monoclonal antibody (Cell Signaling Technology, 8H10D10), goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology, sc-2004), and donkey anti-mouse IgG-HRP (Abcam, ab97030). .. Validation The ATGL antibody was obtained from Cell Signaling Technology and α-Tubulin from Abcam.

    Magnetic Resonance Imaging:

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used ATGL antibody (Cell Signaling Technology, 2138S), α-Tubulin (Abcam, ab7291), goat anti-rabbit antibody (LI-COR Biosciences, 926-68071), donkey anti-mouse antibody (LI-COR Biosciences, 926-32212), ACOT7 rabbit polyclonal antibody (Proteintech, 15972-1-AP), β-Actin mouse monoclonal antibody (Cell Signaling Technology, 8H10D10), goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology, sc-2004), and donkey anti-mouse IgG-HRP (Abcam, ab97030). .. Validation The ATGL antibody was obtained from Cell Signaling Technology and α-Tubulin from Abcam.



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    acot7 rabbit polyclonal antibody  (Proteintech)
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    Proteintech acot7 rabbit polyclonal antibody
    Fig. 1 Identification of convergent evolutionary sites in flight-degenerate birds. a A phylogeny tree5 with American alligator as the outrgoup showing the convergence of eight studied flight-degenerate bird species. b Flying and flight-degenerate species were classified based on previous literatures and observations (Methods). Flight-degenerate species includes flightless birds and weak flyers. Average body mass of each species is plotted against its average wingspan measurement. c Two-dimensional frequency spectrum for nucleotides in flying and flight-degenerate species. The nucleotide frequencies of flight-degenerate birds were plotted against those of flying birds. The x-axis means the nucleotide counts among the eight flight-degenerate bird species and the y-axis means the nucleotide counts among 40 flying bird species. The number of loci is color-coded according to the scale on the right. d The pathway in which ATGL and <t>ACOT7</t> are involved35,70. The main pathway for lipid metabolism is shadowed. In e and f, the upper shows the significant values (Fisher’s exact test) for the representative nucleotides on ATGL e and ACOT7 f, the middle shows the sequence of amino acids, and the bottom shows the schematic domains. The identified convergent sites are highlighted in red. Source data are provided as a Source Data file
    Acot7 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acot7+rabbit+polyclonal+antibody/ACOT7+Antibody/pm31227702-222-14-19
    Average 93 stars, based on 1 article reviews
    acot7 rabbit polyclonal antibody - by Bioz Stars, 2026-09
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    Fig. 1 Identification of convergent evolutionary sites in flight-degenerate birds. a A phylogeny tree5 with American alligator as the outrgoup showing the convergence of eight studied flight-degenerate bird species. b Flying and flight-degenerate species were classified based on previous literatures and observations (Methods). Flight-degenerate species includes flightless birds and weak flyers. Average body mass of each species is plotted against its average wingspan measurement. c Two-dimensional frequency spectrum for nucleotides in flying and flight-degenerate species. The nucleotide frequencies of flight-degenerate birds were plotted against those of flying birds. The x-axis means the nucleotide counts among the eight flight-degenerate bird species and the y-axis means the nucleotide counts among 40 flying bird species. The number of loci is color-coded according to the scale on the right. d The pathway in which ATGL and <t>ACOT7</t> are involved35,70. The main pathway for lipid metabolism is shadowed. In e and f, the upper shows the significant values (Fisher’s exact test) for the representative nucleotides on ATGL e and ACOT7 f, the middle shows the sequence of amino acids, and the bottom shows the schematic domains. The identified convergent sites are highlighted in red. Source data are provided as a Source Data file
    Rabbit Polyclonal Anti Acot7, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acot7+rabbit+polyclonal+antibody/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/10__1194_slash_jlr__m076489-91-6-11
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    Image Search Results


    Fig. 1 Identification of convergent evolutionary sites in flight-degenerate birds. a A phylogeny tree5 with American alligator as the outrgoup showing the convergence of eight studied flight-degenerate bird species. b Flying and flight-degenerate species were classified based on previous literatures and observations (Methods). Flight-degenerate species includes flightless birds and weak flyers. Average body mass of each species is plotted against its average wingspan measurement. c Two-dimensional frequency spectrum for nucleotides in flying and flight-degenerate species. The nucleotide frequencies of flight-degenerate birds were plotted against those of flying birds. The x-axis means the nucleotide counts among the eight flight-degenerate bird species and the y-axis means the nucleotide counts among 40 flying bird species. The number of loci is color-coded according to the scale on the right. d The pathway in which ATGL and ACOT7 are involved35,70. The main pathway for lipid metabolism is shadowed. In e and f, the upper shows the significant values (Fisher’s exact test) for the representative nucleotides on ATGL e and ACOT7 f, the middle shows the sequence of amino acids, and the bottom shows the schematic domains. The identified convergent sites are highlighted in red. Source data are provided as a Source Data file

    Journal: Nature communications

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.

    doi: 10.1038/s41467-019-10682-3

    Figure Lengend Snippet: Fig. 1 Identification of convergent evolutionary sites in flight-degenerate birds. a A phylogeny tree5 with American alligator as the outrgoup showing the convergence of eight studied flight-degenerate bird species. b Flying and flight-degenerate species were classified based on previous literatures and observations (Methods). Flight-degenerate species includes flightless birds and weak flyers. Average body mass of each species is plotted against its average wingspan measurement. c Two-dimensional frequency spectrum for nucleotides in flying and flight-degenerate species. The nucleotide frequencies of flight-degenerate birds were plotted against those of flying birds. The x-axis means the nucleotide counts among the eight flight-degenerate bird species and the y-axis means the nucleotide counts among 40 flying bird species. The number of loci is color-coded according to the scale on the right. d The pathway in which ATGL and ACOT7 are involved35,70. The main pathway for lipid metabolism is shadowed. In e and f, the upper shows the significant values (Fisher’s exact test) for the representative nucleotides on ATGL e and ACOT7 f, the middle shows the sequence of amino acids, and the bottom shows the schematic domains. The identified convergent sites are highlighted in red. Source data are provided as a Source Data file

    Article Snippet: After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat antirabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin).

    Techniques: Sequencing

    Fig. 4 Modeling simulations of effects of functional changes on the energy landscape. The actual abundance of acyl-CoA a and acetyl-CoA b was measured in three flight-degenerate (turkey, chicken and ostrich) and three flying species (zebra finch, parrot and pigeon) using LC–MS. The simulated results for ATGL and ACOT7 are shown in c and d respectively. *P < 0.05, ***P < 0.001 by Wilcoxon rank sum test. Error bars indicate s.e.m. Source data are provided as a Source Data file

    Journal: Nature communications

    Article Title: Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.

    doi: 10.1038/s41467-019-10682-3

    Figure Lengend Snippet: Fig. 4 Modeling simulations of effects of functional changes on the energy landscape. The actual abundance of acyl-CoA a and acetyl-CoA b was measured in three flight-degenerate (turkey, chicken and ostrich) and three flying species (zebra finch, parrot and pigeon) using LC–MS. The simulated results for ATGL and ACOT7 are shown in c and d respectively. *P < 0.05, ***P < 0.001 by Wilcoxon rank sum test. Error bars indicate s.e.m. Source data are provided as a Source Data file

    Article Snippet: After the block with 5% skim milk, the membranes were incubated with primary antibodies (ACOT7 rabbit polyclonal antibody (1:1000, Proteintech, 15972-1-AP) and β-Actin mouse monoclonal antibody (internal control) (1:5000, Cell Signaling Technology, 8H10D10)) overnight at 4 °C followed by the incubation of secondary antibodies (goat antirabbit IgG-HRP (1:10,000, Santa Cruz Biotechnology, sc-2004) for ACOT7, donkey anti-mouse IgG-HRP (1:10,000, Abcam, ab97030) for β-Actin).

    Techniques: Functional Assay, Liquid Chromatography with Mass Spectroscopy